人热休克蛋白-60(Hsp-60)ELISA Kit价格/报价|人ELISA(other)|人ELISA|细胞因子检测试剂盒ELISA

快速查询ID:

127281

品名：

人热休克蛋白-60(Hsp-60)ELISA Kit

目录号：

LSE0822h

供应厂商：

人热休克蛋白-60(Hsp-60)ELISA Kit规格：

96T

人热休克蛋白-60(Hsp-60)ELISA Kit价格：

3680

人热休克蛋白-60(Hsp-60)ELISA Kit说明书：

简单说明：

人热休克蛋白-60(Hsp-60)ELISA Kit英文名：Human Heat Shock Protein,HSP-60 ELISA Kit
预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中人热休克蛋白60（Hsp60）含量。

实验原理
热休克蛋白60（HSP60）是一类进化上高度保守的蛋白质家族。生理状态时协助多肽或蛋白质的正确转位、折叠和装配,，起“分子伴侣”的作用；在应激状态下，HSP60过表达或异位表达,作为一种自身抗原被免疫系统识别，诱发机体的保护性免疫应答，也可作为一种信号分子，在信号转导中发挥作用.近年来研究证实HSP60在自身免疫性疾病、传染病、动脉粥样硬化及慢性感染的发病中均发挥重要的作用。本试剂盒应用双抗体夹心酶标免疫分析法测定标本中HSP60水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HSP60抗原、生物素化的抗人HSP60抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HSP60呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use
This immunoassay kit allows for the specific measurement of human heat shock protein 60 (HSP60) concentrations in cell culture supernates, serum, and plasma.

Introduction
Heat shock protein 60s (hsp60) are remarkably immunogenic, and both T-cell and antibody responses to hsp60 have been reported in various inflammatory conditions.
Analysis of the nucleotide sequences of the TCR demonstrated that human hsp60-reactive T-cell clones and periodontitis lesion-infiltrating T cells have the same receptors, suggesting that hsp60-reactive T cells accumulate in periodontitis lesions. Analysis of the cytokine profile demonstrated that hsp60-reactive PBMC produced significant levels of gamma interferon (IFN-γ) in periodontitis patients, whereas P. gingivalis GroEL did not induce any skewing toward a type1 or type2 cytokine profile. In control subjects no significant expression of IFN-γ or interleukin 4 was induced. These results suggest that periodontitis patients have human hsp60-reactive T cells with a type 1 cytokine profile in their peripheral blood T-cell pools.Heat shock protein 60 (HSP60) is homologous to HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is an abundant protein which is constitutively expressed and is induced by environmental stress. HSP60 exists as a large oligomer composed of 14 ~60 kDa subunits arranged as two stacked rings.

Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for HSP60 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP60 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for HSP60 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP60 bound in the initial step. The color development is stopped and the intensity of the color is measured.