人超氧化物歧化酶(SOD)ELISA Kit价格/报价|人ELISA(other)|人ELISA|细胞因子检测试剂盒ELISA

快速查询ID:

127279

品名：

人超氧化物歧化酶(SOD)ELISA Kit

目录号：

LSE0596h

供应厂商：

人超氧化物歧化酶(SOD)ELISA Kit规格：

96T

人超氧化物歧化酶(SOD)ELISA Kit价格：

3680

人超氧化物歧化酶(SOD)ELISA Kit说明书：

简单说明：

人超氧化物歧化酶(SOD)ELISA Kit英文名：Human Super Oxidase Dimutase,SOD ELISA Kit
预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中SOD含量。

实验原理
超氧化物歧化酶(SOD)是生物体内一种重要的超氧阴离子自由基清除剂，分为Cu,Zn-SOD、Fe-SOD和Mn-SOD 3类，在生物界分布较广，其主要作用是能专一地清除生物氧化中产生的超氧阴离子自由基(O2-.)，有助于减少和阻止脂质的过氧化反应、延缓机体衰老。
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中SOD水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入SOD抗原、生物素化的抗人SOD抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的SOD呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use
This immunoassay kit allows for the specific measurement of Human Superoxide Dismutases (SOD) concentrations in cell culture supernates, serum, and plasma.

Introduction
Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the converversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide.
SOD is a metalloenzyme whose active center is occupied by copper and zinc, sometimes manganese or iron. SOD plays an extremely important role in the protection of all aerobic life-systems, including man, against oxygen toxicity (and the free radicals derived from oxygen).The enzyme superoxide dismutase, or SOD, catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. SOD is an endogenously produced intracellular enzyme present in essentially every cell in the body. There are at least three forms of superoxide dismutase in nature. Human erythrocytes contain an SOD enzyme with divalent copper and divalent zinc. Chicken liver mitochondria and E. coli contain a form with trivalent manganese. E. coli also contains a form of the enzyme with trivalent iron. The Cu-Zn enzyme is a dimer of molecular weight 32,500. The two subunits are joined by a disulfide bond. Superoxide dismutases are enzymes that play major roles in the protection of cells against oxidative damage. The two major forms of superoxide dismutase (SOD) in humans are the mitochondrial manganese SOD and the cytosolic copper/zinc SOD. A copper/zinc SOD, isolated from beef liver, has been used intra-articularly for degenerative joint disorders as an anti-inflammatory agent. SOD is also marketed as a nutritional supplement.Cellular SOD is actually represented by a group of metalloenzymes with various prosthetic groups. The prevalent enzyme is cupro-zinc (CuZn) SOD, which is a stable dimeric protein (32,000 D). SOD is an enzyme associated with copper, zinc, and manganese by body cells, and breaks down the superoxide free radicals. It is said that SOD protects the lens of the eyes by guarding against free radical damage.

Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for SOD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for SOD is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD bound in the initial step. The color development is stopped and the intensity of the color is measured.