人肿瘤排斥性抗原gp96(gp96)ELISA Kit价格/报价|人ELISA(other)|人ELISA|细胞因子检测试剂盒ELISA

快速查询ID:

127278

品名：

人肿瘤排斥性抗原gp96(gp96)ELISA Kit

目录号：

LSE0823h

供应厂商：

人肿瘤排斥性抗原gp96(gp96)ELISA Kit规格：

96T

人肿瘤排斥性抗原gp96(gp96)ELISA Kit价格：

3680

人肿瘤排斥性抗原gp96(gp96)ELISA Kit说明书：

简单说明：

人肿瘤排斥性抗原gp96(gp96)ELISA Kit英文名：Human Glycoprotein,GP-96 ELISA Kit
预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中gp96含量。

实验原理
糖蛋白96(glycoprotein96, gp96)是热应激蛋白家族的一个成员，存在于内质网内膜上。近年研究发现，gp96作为分子伴侣可结合细胞中的肽库。当病毒感染或肿瘤发生时，gp96可与抗原肽结合。随后gp96-抗原肽复合物在一个受体介导的作用下将抗原肽传递给巨噬细胞或其它抗原递呈细胞，经抗原递呈途径，抗原肽被递呈给MHCⅠ类分子，进而激活特异性CTL和记忆性T细胞，引起机体的细胞免疫反应。通常认为，在这个过程中gp96只起抗原传递载体的作用，本身没有发生变化。
本试剂盒应用双抗体夹心酶标免疫分析法测定标本gp96水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入gp96抗原、生物素化的抗人gp96抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的gp96呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use
This immunoassay kit allows for the specific measurement of Human Glycoprotein 96 concentrations in cell culture supernates, serum, and plasma.

Introduction
Heat shock proteins (HSPs), which are important for a number of different intracellular functions, are occasionally found on the surface of cells. The function of heat shock protein on the cell surface is not understood, although it has been shown to be greater in some tumor cells and some virally infected cells. Surface expression of both glycoprotein 96 (gp96) and Hsp70 occurs on tumor cells, and this expression correlates with natural killer cell killing of the cells.
Glycoprotein 96 (gp96), also known as tumor rejection antigen 1 (TRA1), or glucose regulated protein 94 (GRP94), is a cell surface protein that binds adenosine. Thus, gp96 is also called adenotin and it is similar to various stress-induced proteins. The gp96 serves as a natural adjuvant for chaperoning antigenic peptides into the immune surveillance pathways.
Glycoprotein 96 Can Chaperone Both MHC Class I- and Class II-Restricted Epitopes for In Vivo Presentation, but Selectively Primes CD8+ T Cell Effector Function gp96 facilitated the cross-presentation of a class I-restricted peptide and priming of effector function in cognate CD8 cells. Interestingly, gp96 also facilitated the in vivo presentation of a class II-restricted peptide; however, the resulting CD4 cell response did not involve the development of effector function. Taken together, these data suggest that gp96 is an inflammatory mediator that selectively primes CD8 cell effector function.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Glycoprotein 96 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Glycoprotein 96 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Glycoprotein 96 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Glycoprotein 96 bound in the initial step. The color development is stopped and the intensity of the color is measured.