人总溶血补体(CH50)ELISA Kit价格/报价|人ELISA(other)|人ELISA|细胞因子检测试剂盒ELISA

快速查询ID:

127277

品名：

人总溶血补体(CH50)ELISA Kit

目录号：

LSE0813h

供应厂商：

人总溶血补体(CH50)ELISA Kit规格：

96T

人总溶血补体(CH50)ELISA Kit价格：

3680

人总溶血补体(CH50)ELISA Kit说明书：

简单说明：

人总溶血补体(CH50)ELISA Kit英文名：Human total hemolytic complement,CH50 ELISA Kit

预期应用
ELISA法定量测定人的血清、血浆或其它相关液体中CH50含量。

实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CH50水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CH50抗原、生物素化的抗兔CH50抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CH50呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use
This immunoassay kit allows for the specific measurement of Human CH50 concentrations in serum and plasma.

Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for CH50 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CH50 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for CH50 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CH50 bound in the initial step. The color development is stopped and the intensity of the color is measured.