人儿茶酚胺(CA)ELISA Kit价格/报价|人ELISA(other)|人ELISA|细胞因子检测试剂盒ELISA

快速查询ID:

127255

品名：

人儿茶酚胺(CA)ELISA Kit

目录号：

LSE0901h

供应厂商：

人儿茶酚胺(CA)ELISA Kit规格：

96T

人儿茶酚胺(CA)ELISA Kit价格：

3680

人儿茶酚胺(CA)ELISA Kit说明书：

简单说明：

人儿茶酚胺(CA)ELISA Kit英文名：Human catecholamine,CA ELISA Kit

预期应用
    ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中CA含量。
实验原理    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CA水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CA抗原、生物素化的抗人CA抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
    This immunoassay kit allows for the specific measurement of human Catecholamine concentrations in cell culture supernates, serum, and plasma.

Introduction
    Catecholamine (CA), an amine derived from the amino acid tyrosine -- examples include epinephrine (adrenaline), norepinephrine (noradrenaline), and dopamine -- that act as hormones or neurotransmitters. There are a number of disorders involving catecholamines, including neuroblastoma, pheochromocytoma, chemodectina, the familial paraganglioma syndrome, dopamine-ß-hydroxalase deficiency, and tetrahydrobiopterin deficiency.

Test principle
    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Catecholamine has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Catecholamine present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Catecholamine is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Catecholamine bound in the initial step. The color
development is stopped and the intensity of the color is measured.