Endoglycosidase F3, Recomb.价格/报价|new|服务

快速查询ID:

390393

品名：

Endoglycosidase F3, Recomb.

目录号：

LS324727-200MIU

供应厂商：

Merck-Calbiochem

Endoglycosidase F3, Recomb.规格：

200MIU

Endoglycosidase F3, Recomb.价格：

询价

Endoglycosidase F3, Recomb.说明书：

简单说明：

Endoglycosidase F3, Recomb.(Endo-β-N-acetylglucosaminidase F3; Endo F3), Liquid. In 20 mM Tris-HCl, pH 7.5. DO NOT FREEZE. Cleaves asparagine-linked or free biantennary and triantennary complex oligosaccharides. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosyation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on oligomannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat.No. 362185) and therefore is more suitable for deglycosylation of native proteins. <i>Activity: ≥5 units/ml. Specific activity: ≥30 units/mg protein. One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 μmol of denatured porcine fibrinogen per minute at 37℃, pH 5.5. Contaminating enzymes: N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 4.5. (Note: enzyme will precipitate above pH 6.0)</i>. EC 3.2.1.96, M.W. 32000. Note: 1 mU= 1 milliunitITEM TYPE: FG; A