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产 品 库 --> Endoglycosidase F2, Recomb.
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Endoglycosidase F2, Recomb.

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快速查询ID: 390392
品名: Endoglycosidase F2, Recomb.
目录号: LS324726-200MIU
供应厂商: Merck-Calbiochem
Endoglycosidase F2, Recomb.规格:  200MIU
Endoglycosidase F2, Recomb.价格: 询价
Endoglycosidase F2, Recomb.说明书:
简单说明: Endoglycosidase F2, Recomb.(Endo-β-N-acetylglucosaminidase F2; Endo F2), Liquid. In 25 mM NaCl, 10 mM sodium acetate, pH 4.5. DO NOT FREEZE. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat.No. 362185) and therefore is more suitable for deglycosylation of native proteins. <i>Activity: ≥5 units/ml. Specific activity: ≥20 units/mg protein. One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 μmol of denatured porcine fibrinogen per minute at 37℃, pH 5.5. Contaminating enzymes: N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 4.5. (Note: enzyme will precipitate above pH 6.0)</i>. EC 3.2.1.96, M.W. 32000. Note: 1 mU= 1 milliunitITEM TYPE: FG; A
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