Endoglycosidase F1, Recomb.价格/报价|new|服务

快速查询ID:

390391

品名：

Endoglycosidase F1, Recomb.

目录号：

LS324725-700MIU

供应厂商：

Merck-Calbiochem

Endoglycosidase F1, Recomb.规格：

700MIU

Endoglycosidase F1, Recomb.价格：

询价

Endoglycosidase F1, Recomb.说明书：

简单说明：

Endoglycosidase F1, Recomb.(Endo-β-N-acetylglucosaminidase F1; Endo F1), Liquid. In 20 mM Tris-HCl, pH 7.5. DO NOT FREEZE. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat.No. 362185) and therefore is more suitable for deglycosylation of native poteins. <i>Activity: ≥17 units/ml. Specific activity: ≥16 units/mg protein. One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 μmol of denatured Ribonuclease B per minute at 37℃, pH 5.5. Contaminiating enzymes: N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected</i>. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 5.5. EC 3.2.1.96, M.W. 32000. Note: 1 mU= 1 milliunitITEM TYPE: FG; A